Potential of Mitochondria-Targeted Antioxidants to Prevent Oxidative Stress in Pancreatic β-cells

Figure 9

Prevention of palmitic acid-induced mitochondrial network fragmentation in INS-1E cells by SkQ1. 3D superresolution images of the mitochondrial network in mtEos-transfected INS-1E cells preincubated in a medium with 3 mmol·l^-1 glucose were analyzed after 60 min incubation with 150 μmol·l^-1 palmitic acid for the number of fragments. Using the Amira Skeleton algorithm (see inset for the resulting mitochondrial network models), the network was artificially connected, and its whole length was taken as a basic parameter. Subsequently, the number of fragments per unit of length was calculated and plotted. Where indicated, 1 nmol·l^-1 SkQ1 was added at the beginning of a 60 min incubation. The error bars represent the standard error of the mean of 5–10 3D images. ANOVA (–10): .