Research Article
Artemether Activation of AMPK/GSK3β(ser9)/Nrf2 Signaling Confers Neuroprotection towards β-Amyloid-Induced Neurotoxicity in 3xTg Alzheimer’s Mouse Model
Figure 5
Direct relationship between Artemether effects on AMPK/GSK3β(ser9) phosphorylation and neuroprotection towards Aβ1-42-induced apoptotic cell death in PC12 cells. PC12 cells pretreated with 5 μM compound C (AMPK inhibitor) for 30 min, followed by incubation with 1 μM Aβ1-42 in the presence or absence of different concentrations of Artemether. (a) Cell viability was measured by MTT assay. and were considered significantly different. (b) Apoptosis was determined by flow cytometry. (c) Quantitation of the percentage of cell apoptosis. was considered significantly different. (d) Phosphorylation activities of AMPK/GSK3β(ser9). GAPDH was used for normalization. (e, f) Quantitation of phosphorylation activities of AMPK/GSK3β. All results are presented as (); , , and were considered significantly different.
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