Artemether Activation of AMPK/GSK3β(ser9)/Nrf2 Signaling Confers Neuroprotection towards β-Amyloid-Induced Neurotoxicity in 3xTg Alzheimer’s Mouse Model

Figure 7

Artemether treatment decreased brain cortex apoptosis in 3xTg-AD mice. Artemether was administered by intraperitoneal injection, once a day, at low doses of 5 mg/kg and high doses of 20 mg/kg for 4 weeks. (a) The HE (gross morphology), Nissl (neuronal viability), and deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL, apoptosis) staining in brain cortex slices of wild-type mice, compared to 3xTg-AD mice without or treated with low (Arte low) or high (Arte high) dose of Artemether (scale ). Brain cortex area analyzed for Nissl and TUNEL staining is shown in the figure (bregma (1.94 mm), A or B area); five slides per mouse and five mice per treatment group were used for analysis. (b) Western blotting of Bax and Bcl2 protein expression in each group. Three mice per group were used for western blot. (c, d) Quantitation of the Nissl and TUNEL staining data. , , and were considered significantly different.

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