miR-181c-5p Exacerbates Hypoxia/Reoxygenation-Induced Cardiomyocyte Apoptosis via Targeting PTPN4

Figure 6

Transfection of cells with PTPN4 siRNA (PTPN4^KD) resulted in significant reduction of PTPN4 mRNA (a) and protein (b) levels in H9C2 cardiomyocytes. Knockdown of PTPN4 exacerbated the H/R-induced H9C2 cell injury, as evidenced by further increased LDH release (c), reduced cell viability (d), and increased apoptotic cell death as evidenced by increased cleaved caspase 3 (e) and increased TUNEL-positive cells (f). Scale bar: 200 μm. Knockdown of PTPN4 significantly increased the expression of miR-181c-5p (g). Data are shown as ; vs. CTL, vs. scramble siRNA (PTPN4^WT), .

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