Exosomes Derived from TIMP2-Modified Human Umbilical Cord Mesenchymal Stem Cells Enhance the Repair Effect in Rat Model with Myocardial Infarction Possibly by the Akt/Sfrp2 Pathway
Characterization of hucMSCs and hucMSC-derived exosomes. (a) Representative images of spindle-shaped hucMSCs and terminally differentiated adipogenic (Oil Red O-stained) and osteogenic (alizarin A-stained) cells. Scale bar: 50 μm. (b) Flow cytometric plots showing enriched CD90+, CD73+, CD105+, and CD45- hucMSCs. (c) Mass spectrometry results of the TIMP2 level in hucMSC-derived exosomes. (d) Quantification of TIMP2 mRNA and protein expression in TIMP2-transfected hucMSCs. (e) TEM micrographs showing the morphology of hucMSCNC- and hucMSCTIMP2-derived exosomes. Scale bar: 500 nm/200 nm. (f) Immunoblots showing the expression levels of CD9 and CD63 in hucMSCsNC, hucMSCsTIMP2, huc-exoNC, and huc-exoTIMP2. ,, and .
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