Exosomes Derived from TIMP2-Modified Human Umbilical Cord Mesenchymal Stem Cells Enhance the Repair Effect in Rat Model with Myocardial Infarction Possibly by the Akt/Sfrp2 Pathway
huc-exoTIMP2 promoted angiogenesis both in vivo and in vitro. (a) Representative images of heart sections showing in situ expression of CD31 and lectin; quantitative comparison of CD31 and lectin fluorescence intensity. CD31: red, lectin: green, DAPI: blue; scale bar: 50 μm. Blank: rats without surgery; MI+PBS: MI rats treated with PBS; NC: MI rats treated with huc-exoNC; TIMP2: MI rats treated with huc-exoTIMP2. (b) Viability of differentially treated HUVECs. OD540: optical density at 540 nm. (c) Representative images of the wound healing assay showing in vitro migration of HUVECs. Scale bar: 50 μm. (d) Representative images of in vitro tube formation by HUVECs; comparison of tube number and length among different groups. Scale bar: 50 μm. Control: untreated; H2O2: treated with H2O2 alone; NC: treated with huc-exoNC following H2O2; TIMP2: treated with huc-exoTIMP2 following H2O2. ,, and .
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