Exosomes Derived from TIMP2-Modified Human Umbilical Cord Mesenchymal Stem Cells Enhance the Repair Effect in Rat Model with Myocardial Infarction Possibly by the Akt/Sfrp2 Pathway
huc-exoTIMP2 protects the cardiomyocyte against apoptosis induced by MI injury via the Akt pathway. (a) Immunoblots showing the expression level of phosphorylated Akt in the infarcted rat hearts and quantitative comparison of p-Akt levels across different groups. Blank: rats without surgery; MI+PBS: MI rats treated with PBS; NC: MI rats treated with huc-exoNC; TIMP2: MI rats treated with huc-exoTIMP2. (b) Flow cytometry plots showing annexin V/PI-stained cells; comparison of the percentage of apoptotic cells across different groups. (c) Immunoblots showing expression levels of Bax and pro-caspase-9 and comparison of protein expression across different groups. Control: untreated; H2O2: treated with H2O2 alone; TIMP2: treated with huc-exoTIMP2 following H2O2; LY: treated with LY294002 and huc-exoTIMP2 following H2O2. ,, and .
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