Exosomes Derived from TIMP2-Modified Human Umbilical Cord Mesenchymal Stem Cells Enhance the Repair Effect in Rat Model with Myocardial Infarction Possibly by the Akt/Sfrp2 Pathway
The Akt/Sfrp2 pathway mediates the cardioprotective effects of huc-exoTIMP2. (a) Transcriptome sequencing in huc-exoTIMP2 and huc-exoNC. (b) Immunoblots showing the expression level of Sfrp2 in H9C2 cells; quantitative comparison of the protein levels. Control: untreated; TIMP2: treated with huc-exoTIMP2; LY: treated with LY294002 and huc-exoTIMP2. (c) RT-PCR and GFP-fluorescent images presenting the transfection efficiency of shRNA-Sfrp2 in hucMSCsTIMP2. (d) Immunoblots showing the expression levels of Bcl2, Bax, and pro-caspase-9 in H9C2 cells under H2O2 stimulation; quantitative comparison of the protein levels. (e) Representative fluorescent images of TUNEL+ in H9C2 cells with the sh-Sfrp2-exo pretreatment. TUNEL (red) and DAPI (blue). Scale bar: 50 μm. Control: untreated. (f) The quantitative analysis of the ROS level evaluated by flow cytometry using DCFH-DA.sh-NC-exo, treated with sh-NC-exo following H2O2, and sh-Sfrp2-exo, treated with sh-Sfrp2-exo following H2O2. ,, and .
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