CIAPIN1 Targeted NHE1 and ERK1/2 to Suppress NSCLC Cells’ Metastasis and Predicted Good Prognosis in NSCLC Patients Receiving Pulmonectomy

Figure 4

Effect of CIAPIN1 upregulation on NSCLC A549 cells’ metastatic potential was measured. (a) Representative images of cells’ migration and invasion were taken. (b) The relative cells’ migration and invasion after CIAPIN1 upregulation were measured. (c, d) The mRNA expression and the protein levels of MMP2, MMP9, and MMP14 were detected by real-time quantitative PCR and western blotting. (e, f) The mRNA expression and the protein levels of snail, slug, twist, zeb1, and E-cadherin were detected by real-time quantitative PCR and western blotting. (g) Expressions of cell cycle- and apoptosis-associated proteins were detected by western blotting. The expressions of cyclin D1, cyclin D2, cyclin D3, cyclin E, cdk2, and cdk4 were detected in A549 cells with CIAPIN1 overexpression. The expressions of Bax, Bid, Bim, Bcl-2, and Mcl-1 were detected in A549 cells with CIAPIN1 overexpression. GAPDH was used as an internal control. Each value indicated the of three independent experiments. , , compared with control.

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