CIAPIN1 Targeted NHE1 and ERK1/2 to Suppress NSCLC Cells’ Metastasis and Predicted Good Prognosis in NSCLC Patients Receiving Pulmonectomy

Figure 6

ERK1/2 signaling pathway was involved in A549 cells’ metastasis conferred by CIAPIN1. (a) A549 cells with CIAPIN1 overexpression were treated with Cariporide or PD98059, and then cells’ migration and invasion were measured by wound healing and Transwell assays. (b) Changes of A549 cells’ migration and invasion after indicated treatments were detected. (c–e) Cariporide or PD98059 was added to A549 cells with CIAPIN1 overexpression, and the expressions of MMP2, MMP9, and MMP14 were detected. The quantification of MMPs was shown. (f, g) Cariporide or PD98059 was added to A549 cells with CIAPIN1 overexpression, and the expressions of snail, slug, twist, zeb1, and E-cadherin were detected. (h, i) CIAPIN1 upregulation led to the suppression of tumor volume. Tumor volume was calculated for a period of 6 weeks. (j) CIAPIN1 upregulation led to decreased tumor outgrowth analyzed by the tumor growth curve. Each value indicated the of three independent experiments. , , compared with control.

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