SGK1 Attenuates Oxidative Stress-Induced Renal Tubular Epithelial Cell Injury by Regulating Mitochondrial Function

Figure 1

Oxidative stress induces HK-2 cell damage and mitochondrial dysfunction in a time- and dose-dependent manner. (a–c) Cell viability was measured in HK-2 cells treated with increasing doses of hydrogen peroxide (H₂O₂) for different time periods. (d) Cell apoptosis was determined by flow cytometric analysis with annexin-V/PI double-staining. (e) The level of cellular ROS concentration was measured via DCFHDA fluorescence by flow cytometry. (f) A luminescence assay was used to measure the cellular ATP levels. Data are presented as the (). and vs. control.

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