SGK1 Attenuates Oxidative Stress-Induced Renal Tubular Epithelial Cell Injury by Regulating Mitochondrial Function

Figure 2

H₂O₂ time-dependently stimulates the SGK1-dependent signaling pathway in HK-2 cells. HK-2 cells were treated with 250 μM H₂O₂ for different time periods. (a) Representative western blot bands are shown at the top of the corresponding graphs. (b–e) Phosphorylated SGK1 (b), total SGK1 (c), phosphorylated GSK3β (d), and PGC-1α (e) protein levels were examined by western blot analysis. Relative protein levels were normalized to GAPDH and total GSK3β protein levels. Data are presented as the (). and vs. control.

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