NOX2-Dependent Reactive Oxygen Species Regulate Formyl-Peptide Receptor 1-Mediated TrkA Transactivation in SH-SY5Y Cells

Figure 6

FPR1-mediated TrkA transactivation promotes SH-SY5Y cell proliferation, migration, and neurite outgrowth. (a) SH-SY5Y cells were grown in the presence or absence of 0.1 μM N-fMLP and preincubated or not with PTX, or cyclosporin H, or GW441756. The cellular proliferation graph is representative of five independent experiments. Cell count was determined at 24, 48, and 72 h after plating (for all groups, 10⁴ cells/well). (b) Representative images (top) and bar graph quantization (bottom) of SH-SY5Y cell migration from 4 independent experiments. Cells were incubated with 0.1 μM N-fMLP or vehicle in the presence or absence of PTX, or cyclosporin H, or GW441756. Images were acquired at 0, 24, and 48 hours after wound injury (scale bar: 20 μm). compared to unstimulated cells. ^§ compared to N-fMLP-stimulated cells. (c) Representative images (top) and bar graph quantization (bottom) of SH-SY5Y neurite outgrowth from five independent experiments. Neurite length was measured in untreated SH-SY5Y cells (control) or treated with 0.1 μM N-fMLP (N-fMLP) or 100 ng/mL NFG (NGF) up to 48 hours. Neurite length was measured at different times (0, 24, and 48 hours). Arrows show neurite formation. compared to unstimulated cells. ^§ compared to N-fMLP-stimulated cells.

(a)

(b)

(c)