Lipid Profile and Aquaporin Expression under Oxidative Stress in Breast Cancer Cells of Different Malignancies

Figure 3

Antioxidant defense response upon oxidative stress. (a) ROS intracellular accumulation 60 minutes after H₂O₂ treatment. Cells were incubated with a range of H₂O₂ concentrations (10 μM to 100 μM), and ROS accumulation was recorded over 60 min. Data was normalized with basal levels of intracellular ROS. (b) Representative signals of fluorescence output after addition of 100 μM H₂O₂. (c) Nrf2 protein expression of treated (100 μM H₂O₂) vs. nontreated cells, normalized to GAPDH. (d) Nrf2 protein expression of cells treated (+) and nontreated (-) with 100 μM H₂O₂ (representative blots). , , and .

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