Therapeutic Effect of the Mitochondria-Targeted Antioxidant SkQ1 on the Culture Model of Multiple Sclerosis

Figure 1

Determination of the cell types forming the migration zone in the primary oligodendrocyte culture of cerebellar explants upon 10 days of cultivation. (a) Cultured oligodendrocytes of a newborn rat: general view of the culture. The cells radially migrate from the explant of the cerebellum (dark-gray fragment on the right side) in vitro. Bar, 50 μm; transmitted-light microscopy; objective—10x. (b) The field of view within the formed migration zone. The cells were probed with anti-Oligo1 antibody. (c) Myelin synthesis by oligodendrocytes; merged image; probing with both antibody types, anti-Oligo1 to mark oligodendrocytes (red) and antibodies to MBP to mark myelin (green). (d–f) The cells were probed with both types of antibodies, anti-Oligo1 antibody for oligodendrocytes (positive result, red) and anti-beta III tubulin antibody for neurons (negative result, green). (b–f) Bar, 10 μm; confocal microscope, Carl Zeiss LSM 510.

(a)

(b)

(c)

(d)

(e)

(f)