Antioxidant and Anti-Inflammatory Properties of Anthocyanins Extracted from Oryza sativa L. in Primary Dermal Fibroblasts

Figure 2

Effects of ANT on cell viability, migration, and chemotaxis of RDFs. RDFs were treated with or without ANT at 5–25 μg/mL. (a) Cell viability was measured by the MTT assay. (b) Representative figure of CI values for cell migration over 24 h. (c) CI values for ANT at different time points following treatment with varying concentrations of ANT. (d) Boyden chamber assay demonstrating increased migration of RDFs incubated with ANT. Cells were allowed to migrate for 24 h, and then, they were fixed and stained with 10% Giemsa stain. The numbers of migrating cells were averaged from three ×10 field-of-view images. Original magnification ×100. (e) Results were quantified by counting the stained cells and are expressed relative to the mean number of cells randomly migrating in the . The results presented are from three independent experiments; and vs. control.

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