Research Article

Antioxidant and Anti-Inflammatory Properties of Anthocyanins Extracted from Oryza sativa L. in Primary Dermal Fibroblasts

Figure 4

RT-qPCR analysis of the effects of ANT on H2O2-induced mRNA expression in RDFs at 10 h. (a) RDFs were treated with the indicated concentrations of ANT for 2 h, and then, the COL1A2 mRNA levels were quantified. (b) RDFs were treated with the indicated concentrations of ANT for 2 h before the addition of 0.3 mM H2O2. COL1A2 mRNA levels were quantified. Effects of ANT pretreatment on H2O2- or PMA-induced NF-κB p50 (c) and (d) p65 mRNA expression. The relative levels of mRNA were normalized against GAPDH from the same cDNA preparation. Values are presented as the of three independent experiments. and .
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