Antioxidant and Anti-Inflammatory Properties of Anthocyanins Extracted from Oryza sativa L. in Primary Dermal Fibroblasts

Table 1

Primer sequences used for RT-qPCR.

Target gene	Forward primer	Reverse primer	Amplicon size (bp)	Accession number
COL1A2	5-ACCTCAGGGTGTTCAAGGTG-3	5-CGGATTCCAATAGGACCAGA-3	222	NM_053356.1:1
NF-κB p50	5-AGAGGATGTGGGGTTTCAGG-3	5-GCTGAGCATGAAGGTGGATG-3	200	NM_001276711.1
NF-κB p65	5-CGCCACCGGATTGAAGAAA-3	5-TTGATGGTGCTGAGGGATGT-3	194	LC 369719.1
IL-6	5-GCCCTTCAGGAACAGCTATGA-3	5-TGTCAACAACATCAGTCCCAAGA-3	80	NM_012589.2
GAPDH	5-CCCCCAATGTATCCGTTGTG-3	5-TAGCCCAGGATGCCCTTTAGT-3	118	NM_017008.4

NF-κB: nuclear factor kappa-B; COL1A2: collagen type I alpha 2; IL-6: interleukin-6; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.