Berberine Ameliorates Doxorubicin-Induced Cardiotoxicity via a SIRT1/p66Shc-Mediated Pathway

Figure 3

Effects of Ber on p66Shc and SIRT1 expression in vivo and in vitro as analysed by a Western blot analysis and quantified by a densitometric analysis. (a) Western blot analysis of cardiac p66Shc and SIRT1 protein levels in rats: (A) representative blots of p66Shc expression; (B) representative blots of SIRT1 expression. ^△△ vs. control group, ^## vs. DOX group. (b) Western blot analysis of cardiac p66Shc and SIRT1 protein levels in H9c2 cells treated with DOX, Ber+DOX, or Ber+DOX+EX527. H9c2 cells were treated with or without 10 μM EX527 for 60 min, exposed to 1 μM Ber for 24 h, and then exposed to 1 μM DOX for 24 h: (A) representative blots of p66Shc expression; (B) representative blots of SIRT1 expression. The results are presented as the (). ^△△ vs. control group, ^## vs. DOX group, and vs. Ber+DOX group.

(a)

(b)