Niacin Protects against Butyrate-Induced Apoptosis in Rumen Epithelial Cells

Figure 3

Effects of butyrate and niacin on the intracellular reactive oxygen species (ROS) and the intracellular total antioxidant capacity (T-AOC) of rumen epithelial cells. Rumen epithelial cells were exposed to 120 mM butyrate with or without 40 mM niacin for 6 h. Intracellular ROS levels were measured using 2,7-dichlorodihydro-fluorescein-diacetate (DCFH-DA). Intracellular T-AOC was estimated using a commercial kit according to the manufacturer’s protocol. Data are presented as the (SD). Values with different letters indicate significant differences ().