Research Article
Niacin Protects against Butyrate-Induced Apoptosis in Rumen Epithelial Cells
Table 1
PCR amplification primer design.
| | Genes | Primer sequence | Origin | Size (bp) | Annealing temperature (°C) |
| | GADPH | F: 5-AGGTTGTCTCCTGCGACTTCA-3 | NM001190390.1 | 132 | 84.59 | | R: 5-CCCTGTTGCTGTAGCCGAAT-3 | | Fas | F: 5-CTCTGAGGGGCTGAGATTGA-3 | NM001123003.1 | 107 | 82.44 | | R: 5-GTTTGCCAGGAGGACAAGG-3 | | Bcl-2 | F: 5-TGTTTGATTTCTCCTGGCTGT-3 | XM012103831 | 145 | 86.69 | | R: 5-ACTGCTTTCACGAACCTTTTG-3 | | Bax | F: 5-TTCCGACGGCAACTTCAAC-3 | XM015100639.1 | 244 | 88.29 | | R: 5-GAGCACTCCAGCCACAAAGA-3 | | Caspase-3 | F: 5-GCAGCAAACCTCAGGGAAA-3 | XM15104559.1 | 154 | 80.98 | | R: 5-CATGGCTTAGAAGCACGCA-3 | | Caspase-9 | F: 5-TGTTGCCGTTTCCTTCTCC-3 | XM015099300.1 | 111 | 84.35 | | R: 5-CTAGCACTTCGCTTTCTGGTG-3 | | Caspase-8 | F: 5-AAAATGCCCTTCCCTTGTTG-3 | XM012142500.2 | 110 | 80.95 | | R: 5-CTTCCCTCTGTTCTGAGTCGGT-3 | | PARP-1 | F: 5-CTCCAATCGCTTCTACACCC-3 | XM_012118480.2 | 49 | 86.07 | | R: 5-AACCACCCCTGAGTAGACTGTAG-3 | | P53 | F: 5-CAGGAGACATTTTCCGACTTG-3 | NM001009403.1 | 122 | 83.89 | | R:5-TCATCCAGCCAGGTGACAA-3 |
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