Inhibitor 1 of Protein Phosphatase 1 Regulates Ca²⁺/Calmodulin-Dependent Protein Kinase II to Alleviate Oxidative Stress in Hypoxia-Reoxygenation Injury of Cardiomyocytes

Figure 5

I1PP1 overexpression inhibited ROS but elevated mitochondrial membrane potential in cardiomyocytes after H/R injury. After infection of the I1PP1 recombinant adenovirus, the cardiomyocytes were subjected to hypoxia for 4 h followed by 12 h reoxygenation. (a) Superoxide production in the cardiomyocytes was detected using a fluorescence microscope with DHE fluorescent probe and quantified using ImageJ analysis software. . (b) Mitochondrial ROS was measured using MitoSOX. Mitochondrial localization of MitoSOX signal was confirmed by colocalization with MitoTracker Green and quantified using ImageJ analysis software. . (c) Mitochondrial membrane potential (Δψm) was measured by JC-1 staining and quantified using ImageJ analysis software. . Plots represent the ; . Statistical significance: compared with the control; ^## compared with H/R.

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