Lipoxin A4 Ameliorates Acute Pancreatitis-Associated Acute Lung Injury through the Antioxidative and Anti-Inflammatory Effects of the Nrf2 Pathway

Figure 2

LXA4 inhibited proinflammatory cytokine and ROS generation in AP-ALI mice. Mouse serum and lung tissue samples were collected to evaluate injury 24 h after modeling. (a–c) The serum levels of the inflammatory factors TNF-α, IL-1β, and E-selectin were measured by ELISA. (d) Immunohistochemistry micrographs of lung tissue sections show the expression of IL-6. (e) Flow cytometric analysis of the cellular ROS in lung tissues in different experimental mouse groups was performed. (f) The quantitative mean fluorescence intensity in each treatment group is shown. and vs. the control group. ^# and ^## vs. the AP group. AP: the acute pancreatitis group; AP+LXA4: the acute pancreatitis+Lipoxin A4 group; AP-ALI: the acute pancreatitis-induced acute lung injury; ROS: reactive oxygen species.

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