Lipoxin A4 Ameliorates Acute Pancreatitis-Associated Acute Lung Injury through the Antioxidative and Anti-Inflammatory Effects of the Nrf2 Pathway

Figure 4

LXA4 activated the Nrf2 pathway and upregulated HO-1 expression. (a) The total protein levels of Nrf2, pSer40-Nrf2, and HO-1 in HPMECs were determined by Western blotting. β-Actin was used as an internal control. (b) Relative band densities were quantified, and the normalized values are indicated in the histogram. (c) The nuclear Nrf2 and pSer40-Nrf2 levels were assessed by Western blot analysis. For the internal control, Lamin B was used. (d) The blots were analyzed by densitometry, and the results are expressed in the histogram. (e) Nrf2 nuclear translocation in HPMECs was determined using immunofluorescence analysis. (f) A luciferase reporter assay showed that Nrf2 was the gene that most effectively regulated HO-1. All experiments were performed at least three times. , and vs. the control group. # and ## vs. the TNF-α group. TNF-α: the TNF-α group; TNF-α+LXA4, the TNF-α+Lipoxin A4 group; Nrf2: the nuclear factor E2-related factor 2; p-Nrf2: phosphorylated Nrf2; HO-1: heme oxygenase-1; AP-1: activator protein-1; NF-κB: nuclear factor kappa B.

(a)

(b)

(c)

(d)

(e)

(f)