Mitochondria-Targeted Peptide SS31 Attenuates Renal Tubulointerstitial Injury via Inhibiting Mitochondrial Fission in Diabetic Mice

Figure 5

Effects of SS31 on mitochondrial morphology in the kidney of diabetic mice and mitochondrial ROS and mitochondrial membrane potential in HK-2 cells exposed to HG after SS31 administration. (a) EM analysis showed that the diabetic mouse renal tissues displayed obvious mitochondrial morphological changes; these changes were reversed by SS31 treatment (magnification ×5,000). (b) Representations of mitochondrial ROS levels (upper panel) and mitochondrial membrane potential (MMP, bottom panel) in HK-2 cells exposed to HG treatment with SS31 or Mdivi1 pretreatment (magnification ×400). (c) Relative percentages of fragmented mitochondria in the four groups. vs. STZ groups, vs. control groups, . (d, e) Quantification of mitochondrial ROS production as measured with MitoSox Red staining (d) and MMP as measured with TMRE staining (e). vs. HG groups, vs. LG groups, .

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