Increased Transfection of the Easily Oxidizable GC-Rich DNA Fragments into the MCF7 Breast Cancer Cell

Figure 1

(a) Scheme of the human ribosomal repeat. Segments—the inserts of the model GC-DNA analyzed are shown. (b) (1)—plasmid pEGFP-C1 (pEGFP) (http://www.bdbiosciences.com, GenBank accession number U55763). The site BamH1 is marked. (2) and (3)—distribution of the easily oxidizable GGG-motifs within pBR322, pBR322-rDNA, pEGFP, and pEGFP-rDNA. (c) Determination of the plasmid fragmentation in the extracellular DNA after 1 h of MCF7 incubation with 50 ng/mL pEGFP or pEGFP-rDNA. The total DNA was isolated from the cell culture medium. Electrophoresis of DNA was carried out in a 2% agarose gel stained with ethidium bromide. Gel tracks 1 and 2—plasmid solutions in water were applied.

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