Increased Transfection of the Easily Oxidizable GC-Rich DNA Fragments into the MCF7 Breast Cancer Cell

Figure 5

Expression of NOX4 in MCF7. (a) FCA. (1)—the distribution of FL2-NOX4 fluorescence intensities; black: background fluorescence (PE-conjugated secondary antibodies). Cells plots: FL2 (NOX4-PE) versus SSC; R1 and R2: gated areas. (2)—relative fractions of NOX4-positive cells in R1 gate. (3)—median signal intensity of FL2-NOX4 in R1 gate. (b) RT-qPCR. The levels of NOX4-encoding RNAs in the cells exposed to GC-DNA for 24 h. The data are normalized by the content of RNA NOX4 in the control. (c) FM-based evaluation of NOX4 (PE) in the cells treated with pEGFP-rDNA for 24 h (×40). against control group of cells (nonparametric test).

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