Antagonizing Effects of Clematis apiifolia DC. Extract against Benzo[a]pyrene-Induced Damage to Human Keratinocytes

Figure 1

CAE suppresses benzo[a]pyrene (B[a]P) effects on xenobiotic response element- (XRE-) mediated signaling in HaCaT cells. (a) HaCaT cells were transfected with the XRE-Luc reporter and a Renilla-luciferase plasmid using the DharmaFECT® Duo transfection reagent. After 24 h, the cells were treated and incubated with CAE for 14 h in the presence or absence of B[a]P. The cells were then harvested and subjected to a luciferase activity assay. vs. B[a]P-treated control. The results were confirmed by repeating three independent experiments. Data are expressed as the (b, c) HaCaT cells were incubated with CAE for 24 h in the presence or absence of B[a]P (15 μM) and then subjected to Western blot analysis (b) and real-time PCR analysis (c) for CYP1A1. The results were confirmed by repeating the three independent experiments. Data are expressed as the vs. B[a]P-treated control. (d, e, g) HaCaT cells were incubated with CAE (100 μg/ml) or dasatinib in the presence of B[a]P (15 μM) for 24 h and then subjected to Western blot analysis after preparation of the nuclear and cytoplasmic fractions. (f) HaCaT cells were incubated with dasatinib (100 nM) in the presence of B[a]P (15 μM) for 24 h and then subjected to Western blot analysis. CAE: Clematis apiifolia DC.

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