Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 3

Research Article

Relation of Redox and Structural Alterations of Rat Skin in the Function of Chronological Aging

Figure 3

Expression of proliferating cell nuclear antigen (PCNA) in the skin of 3-day-, 15-day-, 1-month-, 3-month-, 6-month-, and 21-month-old rats. Tissues were subjected to immunohistochemistry using a PCNA-specific antibody. The brown nuclei testify to the specific staining (a). The quantitative evaluation of PCNA antibody staining intensity in skin sections was analyzed by the IHC Profiler using the open-source ImageJ program. The relative mean intensity was determined from 6 images for each aging group (), and it is shown graphically (b). Data were presented as . In respect to 3-month-old rats; ; . ×100 magnification, orig.; corner insets show low magnification (×40, orig.) of representative skin sections.