p53-Mediated PI3K/AKT/mTOR Pathway Played a Role in Ptox^Dpt-Induced EMT Inhibition in Liver Cancer Cell Lines

Figure 1

The Topo II inhibition and interaction of Ptox^Dpt. (a) Topo II inhibition of Ptox^Dpt. 1: pUC18; 2: nucleic extract plus pUC18; 3: pUC18 and nucleic extract plus 75 μM Ptox^Dpt; 4: pUC18 and nucleic extract plus 50 μM Ptox^Dpt; 5: pUC18 and nucleic extract plus 500 μM etoposide; 6: pUC18 and nucleic extract plus DMSO. CL = cleaved; L = linear; S = supercoiled; R = relaxed DNA. (b) Ptox^Dpt docked into the Topo II DNA complex. (c) The interactions between Ptox^Dpt and nearby residues. The dark green dash lines indicated hydrogen bonds. The eye-like curved dark red lines represented the hydrophobic residues in contact.

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