Research Article

Interactions between Cytosolic Phospholipase A2 Activation and Mitochondrial Reactive Oxygen Species Production in the Development of Ventilator-Induced Diaphragm Dysfunction

Figure 4

Ventilator decreased diaphragm perfusion. (a) Laser Doppler for diaphragm perfusion; (b) laser Doppler for soleus perfusion; (c) effects of CDIBA and MitoTEMPO on diaphragm perfusion after 12 hours of MV; (d) western blots for HIF-1α in the diaphragm and soleus. MV = mechanical ventilation; cPLA2 = cytosolic phospholipase A2; CDIBA = 4-{2-[5-chloro-1-(diphenylmethyl)-2-methyl-1H-indol-3-yl]-ethoxy} benzoic acid, a specific cPLA2 inhibitor; MitoTEMPO = a mitochondria-targeted antioxidant. and vs. the control group; vs. basal.