Acetaldehyde Induces Neurotoxicity In Vitro via Oxidative Stress- and Ca²⁺ Imbalance-Mediated Endoplasmic Reticulum Stress

Figure 2

The exposure of ADE induces morphological changes of the HT22 cells. HT22 cells were incubated with ADE (0, 0.625, 1.25, and 2.5 mM) for 24 h, and then stained with Hoechst 33258 (a) and AO-EB (b), respectively. . (c) Annexin V-FITC/PI double-staining of cells with ADE (0, 0.625, 1.25, and 2.5 mM) treatment for 24 h. (d) Quantified apoptotic rate (). (e) The expression levels of caspase family proteins, Bax and Bcl-2, by western blot. (f) Densitometric analysis of apoptosis-related proteins (, , vs the control group). Statistical comparisons were made using one-way ANOVA with Dunnett’s multiple comparison tests.

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