NRF1 and NRF2 mRNA and Protein Expression Decrease Early during Melanoma Carcinogenesis: An Insight into Survival and MicroRNAs

Table 3

Primers used in qPCR.

RNA target	Product company	Primer sequences	Amplicon length (base pairs)	Function related to oxidative stress; (references)
mRNA NRF2	Sigma	Forward: 5-CAATGAGGTTTCTTCGGCTACG-3; Reverse: 5-AAGACTGGGCTCTCGATGTG-3	72	Major redox response regulator [1]
mRNA NRF1	Sigma	Forward: 5-ATGGAAATGCAGGCCATGGAAG-3; Reverse: 5-GAGGGGCACTGTACAGGATTT-3	61	Redox response regulator [5]
GAPDH	Sigma	Forward: 5-TGGAAGGACTCATGACCACA-3; Reverse: 5-CCATCACGCCACAGTTT-3		—
miR-23B-3p	Qiagen	5AUCACAUUGCCAGGGAUUACC		Predicted NRF2 inhibition [40]
miR-93-5p	Qiagen	5CAAAGUGCUGUUCGUGCAGGUAG		Predicted NRF2 inhibition [40]
miR-144-3p	Qiagen	5UACAGUAUAGAUGAUGUACU		Predicted NRF2 inhibition [8, 40, 52]
miR-212-3p	Qiagen	5UAACAGUCUCCAGUCACGGCC		NRF1 and NRF2 inhibition, interaction with Mn-SOD
miR-340-3p	Qiagen	5UCCGUCUCAGUUACUUUAUAGC		MAPK signalling, predicted NRF1 inhibition [40]
miR-383-5p	Qiagen	5AGAUCAGAAGGUGAUUGUGGCU		No predicted inhibition of NRF1/NRF2
miR-510-5p	Qiagen	5UACUCAGGAGAGUGGCAAUCAC		No predicted inhibition of NRF1/NRF2
RNU-6B	Qiagen	(Not reported, product no. 218300 cat. no. MS00014000)		—