Research Article
Nrf2/Keap1/ARE Signaling Mediated an Antioxidative Protection of Human Placental Mesenchymal Stem Cells of Fetal Origin in Alveolar Epithelial Cells
Figure 5
The protective role of hfPMSCs in A549 cells from cell apoptosis of H2O2-induced cell oxidative injury. The A549 cells were injured with 600 μmol/L H2O2 prior to being treated with 100 μmol/L VC or cocultured with hfPMSCs (/well) (group 1), or A549 cells (/well) were added with 100 μmol/L VC or cocultured with hfPMSCs (/well) for 24 h, then the medium of the bottom was changed with 600 μmol/L H2O2 for 24 h (group 2). The cell apoptosis was analyzed by a cytometric assay using an Annexin V-FITC/propidium iodide (PI) apoptosis detection kit. Fractions of apoptotic cells in A549 cells treated with normal medium, 100 μmol/L VC, and hfPMSCs in group 1 (top panel) and in group 2 (bottom panel). (c, d) Analysis of apoptotic cell rates in (a), group 1 (c), and group 2 (d), respectively. Data represented the from three independent triplicated experiments (, -test). Compared with the injury group, and represent and , respectively.
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