Cyclophilin A Protects Cardiomyocytes against Hypoxia/Reoxygenation-Induced Apoptosis via the AKT/Nox2 Pathway

Figure 2

CyPA inhibits apoptosis of H9c2 cells under hypoxia/reoxygenation conditions. H9c2 cells were divided into five groups, control (cultured for 22 h under normoxic conditions), H/R (cultured for 18 h of hypoxia followed by 4 h of reoxygenation), H/R+CyPA (1000 ng/mL), H/R+GSK690693 (10 μM), and H/R+CyPA (1000 ng/mL)+GSK690693 (10 μM). The protein expression levels of BAX, Bcl-2, and caspase-3 are revealed in the representative western blot gel files and summarized data (a–c, e). The apoptotic populations of H9c2 cells sorted by flow cytometry after double staining with FITC-Annexin V and propidium iodide (PI) are revealed in representative plots (d) and summarized data (f). vs. the H/R+CyPA group ().

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