Excessive Oxidative Stress Contributes to Increased Acute ER Stress Kidney Injury in Aged Mice

Figure 4

ER stress-induced severe oxidative stress in the kidneys of old mice is prevented by antioxidants. Renal tissue was obtained from young and old mice at baseline, 72 hours after tunicamycin (0.8 μg/g) injection and old mice pretreatment with BHA for 7 days or NAC 24 hours before tunicamycin injection. ER stress caused elevation in MDA, oxidized protein, and AGEs and decreased reducing glutathione (GSH)/glutathione disulfide (GSSG). (a) Levels of MDA, (b) levels of oxidized protein, (c) levels of AGEs, and (d) the ratio of GSH and GSSG. Y: young mice; O: old mice; Y/tuni: young mice with tunicamycin injury; O/tuni: old mice with tunicamycin injury: NAC; old mice treated with NAC; BHA: old mice treated with BHA. and vs. young mice at baseline; vs. old mice at baseline; and vs. old mice that received tunicamycin; and vs. old mice treated with NAC.

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