Oxidative Medicine and Cellular Longevity / 2019 / Article / Fig 2

Research Article

Upregulation of UCP2 Expression Protects against LPS-Induced Oxidative Stress and Apoptosis in Cardiomyocytes

Figure 2

The effect of UCP2 knockdown on LPS-induced cardiomyocyte damage and oxidative stress. Cardiomyocytes were transduced with Ad-shRNA-NC or Ad-shRNA-UCP2 adenovirus followed by treatment with or without 25 μM LPS. (a) Cardiomyocyte damage was determined using the LDH assay. (b, c) The levels of MDA (b) and SOD (c) in the cardiomyocytes were measured using the corresponding kits. (d) ROS production was determined by assessing representative immunofluorescence images. (e, f) Cardiomyocytes were stained with DHE followed by flow cytometry. Data were collected from three individual experiments and presented as the . vs. shRNA-NC, vs. shRNA-NC+LPS.
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