Upregulation of UCP2 Expression Protects against LPS-Induced Oxidative Stress and Apoptosis in Cardiomyocytes

Figure 4

The effect of UCP2 knockdown on LPS-induced cardiomyocyte apoptosis. Cardiomyocytes were transduced with Ad-shRNA-NC or Ad-shRNA-UCP2 adenovirus followed by treatment with or without 25 μM LPS. (a) Cells were stained with Annexin V-FITC and PI followed by flow cytometric analysis. (b) The change in apoptosis rate. (c, d) Effects of UCP2 downregulation on the protein levels of Bax, bcl-2, cleaved-caspase-3 (c), cytochrome c (d), and GAPDH were assessed using western blotting. (e-i) Quantification relative to GAPDH level. Data were collected from three individual experiments and presented as the . vs. shRNA-NC, vs. shRNA-NC+LPS.

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