Morinda citrifolia and Its Active Principle Scopoletin Mitigate Protein Aggregation and Neuronal Apoptosis through Augmenting the DJ-1/Nrf2/ARE Signaling Pathway

Figure 2

Rotenone-induced aggregation of α-synuclein (α-Syn) measured by immunofluorescence analysis. (a) The cells were visualized using fluorescence microscopy and images captured using 20x magnification. The control and sham groups show very low level of expression; however, the expression is punctate and high in the rotenone-induced group; the expression is meager in levodopa; on the contrary, expression in the MCE groups is comparable with that in the control group. (b) Relative fluorescence intensity was calculated. (c) Immunoblot analysis of α-Syn and representative densitometry quantification. Statistical significance () was calculated by Student-Newman-Keuls and least significant difference post hoc test, where represents control vs. other groups, represents rotenone vs. LD, MCE.

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