Morinda citrifolia and Its Active Principle Scopoletin Mitigate Protein Aggregation and Neuronal Apoptosis through Augmenting the DJ-1/Nrf2/ARE Signaling Pathway

Figure 5

(a) Rotenone-induced apoptosis is counteracted by scopoletin in SH-SY5Y cells. At the end of the experiment, cells were stained with annexin V/FITC and read immediately by flow cytometry to measure the extent of apoptosis; -axis FL-1H denotes the FITC and -axis FL-2H denotes the PI. (b) Quantification of percentage dead cells. (c) Immunoblot analysis of several proteins involved in Nrf2/ARE pathway is performed in SH-SY5Y cells treated with/without rotenone and scopoletin. (d) Immunoblot analysis of phospho (S40) Nrf2 and nuclear Keap1 in SH-SY5Y cells. Statistical significance () was calculated by Student-Newman-Keuls and least significant difference post hoc test, where represents control vs. other groups, represents rotenone vs. scopoletin.

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