Ventilation-Like Mechanical Strain Modulates the Inflammatory Response of BEAS2B Epithelial Cells

Figure 4

Extracellular IL-8 levels. IL-8 ELISA readings in culture medium from BEAS2B cells. (a) Nonstimulated controls () and cells subjected to sinusoidal (), rectangular (), or triangular () cyclic strain for 4 h. vs. control; vs. rectangular strain profile. (b) Nonstimulated controls () and cells subjected to cyclic strain () with 20% elongation maximum and elongation minimum (Emin) set to 0, 5, 10, or 15%, respectively. vs. control; vs. Emin 0. (c) Nonstimulated controls () and BEAS2B cells subjected to static strain (Stat; ) of 15 or 20%. vs. control; vs. 15%-Stat. (d) Nonstimulated controls () or BEAS2B cells subjected to strain () resembling conventional ventilation without (Vent-like) or with flow-controlled ventilation (Vent+FLEX-like). Box plots indicate median, upper/lower quartile, and bars with minimal/maximal values (or median and range for ). Statistics: analysis of variance (Tukey’s post hoc test). vs. control; vs. Vent-like strain profile. Bars indicate . All strain profiles are presented in more detailed manner in supplementary figures S1-S3.

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