Protective Effect of Dinitrosyl Iron Complexes with Glutathione in Red Blood Cell Lysis Induced by Hypochlorous Acid

Figure 5

Interaction between hypochlorous acid and DNIC with glutathione ligands (DNIC-GS). The EPR signal recorded in a reaction medium containing 100 mM Na,K-phosphate buffer (pH 7.4) and 1.8 mM DNIC with glutathione ligands (a). The ratio of iron and glutathione under these conditions was approximately 1 : 2.2. The EPR spectra were recorded 2.5 min after the addition of different concentrations of HOCl (b–d). The kinetics of formation of DNIC with phosphate ligands was recorded in a reaction medium containing 1.8 mM DNIC-GS and 8 mM HOCl (e).