Protective Effect of Dinitrosyl Iron Complexes with Glutathione in Red Blood Cell Lysis Induced by Hypochlorous Acid

Figure 7

The reaction medium contained 100 μM DNIC with glutathione (DNIC-GS) or 200 μM GSH in PBS (pH 7.4) and different concentrations of HOCl. The incubation was carried out at ambient temperature (~25°C). The fluorescence spectra were recorded 3 min after the addition of ThioGlo 1 to the cell samples. The kinetics of oxidation of thiol groups of glutathione in the presence of 100 (□), 200 (○), and 400 μM (×) HOCl (a). The kinetics of oxidation of thiol groups in DNIC glutathione ligands in the presence of 100 (■), 200 (●), and 400 μM (▼) HOCl (b); the concentration of thiol groups in GSH (∆) and DNIC-GS (♦) after 10 min incubation with different concentrations of HOCl (c). The structural formula of ThioGlo 1 (d). The fluorescence of the samples prior to the addition of HOCl to the reaction medium was taken for 100%.

(a)

(b)

(c)

(d)