New Insights into Chronological Mobility of Retrotransposons In Vivo
Replicative senescence of MEFs. (a, b) SA-β-gal staining of MEFs, comparing young (P2-MEF, in (a)) and senescent (P7-MEF, in (b)). (c) Quantification of β-gal-positive (blue stained) MEFs. (d) mRNA expression level of Mki67 and P16INK4a examined by RT-qPCR. (e) P16INK4a protein level in young and senescent MEFs. β-Tubulin was used as the loading control. (f) RT-qPCR analysis of different classes of RTEs. ,, and .
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