Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid

Figure 2

Effects of 15s-HpETE on the redox state of PTEN in C2C12, HeLa, and HT22 cells. The cells were treated with 10 μM 15s-HpETE for the indicated times. Cellular protein extracts were then alkylated with 10 mM NEM and subjected to nonreducing or reducing SDS-PAGE, followed by Western blot analysis using antibodies to PTEN.

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