Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid

<div>Effects of 15s-HpETE on the redox state of PTEN in Prx III<svg height="12.308pt" id="M8" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -12.2582 15.6781 12.308" width="15.6781pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M556 236V289H337V504H275V289H56V236H275V-4H337V236H556Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,5.56,-5.741)"><path d="M381 704H317L48 -163H112L381 704Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,9.468,-5.741)"><path d="M556 236V289H337V504H275V289H56V236H275V-4H337V236H556Z"></path></g></svg> and Prx III<svg height="12.308pt" id="M9" style="vertical-align:-0.04979992pt" version="1.1" viewbox="-0.0498162 -12.2582 15.6781 12.308" width="15.6781pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.0091,0,0,-0.0091,0,-5.741)"><path d="M556 236V289H56V236H556Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,5.56,-5.741)"><path d="M381 704H317L48 -163H112L381 704Z"></path></g><g transform="matrix(.0091,0,0,-0.0091,9.468,-5.741)"><path d="M556 236V289H56V236H556Z"></path></g></svg> MEF cells. (a) MEF cells were treated with the mixture of 10 <i>μ</i>M 15s-HpETE and Lipofectamine 2000 transfection reagent for the indicated times. Cellular protein extracts were alkylated with 10 mM NEM and subjected to nonreducing or reducing SDS-PAGE, followed by Western blot analysis using antibodies to PTEN or actin. (b) The intensity of oxidized PTEN was quantitated using ImageJ software.</div>

Oxidative Medicine and Cellular Longevity

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Figure 5

Figure 5: Peroxiredoxin III Protects Tumor Suppressor PTEN from Oxidation by 15-Hydroperoxy-eicosatetraenoic Acid 