RNA-seq Reveals Dysregulation of Novel Melanocyte Genes upon Oxidative Stress: Implications in Vitiligo Pathogenesis

Figure 2

(a) MDS plot: multidimensional scaling plot shows the clustering of controls and H₂O₂ treated samples. A large disparity in expression of genes between controls and H₂O₂ treated with various doses and time points was clearly evident. (b) Volcano plot: the transcriptome of cells treated with 250 μM H₂O₂ for 48 h was compared to control. The -axis represents the log₂ fold changes (log₂FC), and the -axis specifies the statistical significance (negative logarithm to the base 10 of FDR) of differential expression. Green vertical and horizontal lines reflect the filtering criteria ( and ). Red and blue dots represent up- and downregulated genes, respectively. (c) The Venn diagram shows the number of genes upregulated (Red) or downregulated (blue) by treatment with H₂O₂ for 6 h (a), 24 h (b), and 48 h (c) compared to controls. The numbers of differentially regulated genes in two or three groups are shown in intersections.

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