RNA-seq Reveals Dysregulation of Novel Melanocyte Genes upon Oxidative Stress: Implications in Vitiligo Pathogenesis

Figure 4

(a) PIG1 cells and primary HEM were treated with 250 μM H₂O₂ for 48 h, and cell viability was measured by trypan blue dye exclusion assay. (b) PIG1 and primary HEM cells were treated as in (a), and mRNA extracted. Relative gene expression of indicated genes was measured using quantitative RT-PCR. GAPDH was used as internal control. (c) PIG1 cells and primary HEM were treated as in (a); cell lysates were collected and probed with indicated antibodies using western blotting. (d) The relative expression levels induced by treatment with 250 μM H₂O₂ for 48 h of BH family proapoptotic (blue) and antiapoptotic (green) are shown. Only those members whose value is <0.05 are shown.

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