Role of Combined Lipoic Acid and Vitamin D3 on Astrocytes as a Way to Prevent Brain Ageing by Induced Oxidative Stress and Iron Accumulation

Figure 7

Cell viability, ROS production, and iron quantification in astrocytes: (a) cell viability, (b) ROS production, and (c) the intracellular iron quantification measured on astrocytes pretreated with Fe³⁺ for 6 days and then with vitD and LA alone or combined for additional 6 days. The abbreviations are the same as reported in Figure 1. Data are expressed as (%) of five independent experiments normalized to control values (0% line). vs. control; vs. LA; vs. vitD; vs. Fe³⁺, bars: between treatments.

(a)

(b)

(c)