Role of Combined Lipoic Acid and Vitamin D3 on Astrocytes as a Way to Prevent Brain Ageing by Induced Oxidative Stress and Iron Accumulation

Figure 8

Analysis of the main intracellular pathways analyzed by Western blot and the ELISA kit. In (a), (b), and (d), the measurements of p53, APP, and ERK/MAPK activities, respectively, are reported by the ELISA test. Data are expressed as (%) of five independent experiments normalized to control values (0% line). (c) Densitometric analysis and Western blot of cytochrome SOD3 expression obtained in whole astrocyte lysates. Data are expressed as (%) of five independent experiments normalized and verified on β-actin detection. The abbreviations are the same as reported in Figure 1. vs. control; vs. LA; vs. vitD; vs. Fe³⁺, bars: between treatments.

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